Ku-chromatography ephezulu ye-chromatography (HPLC), ukunemba nokusebenza kahle kubalulekile ekutholeni imiphumela efanele. Enye yezinto ezibucayi ekuqinisekiseni ukusebenza okufanele kohlelo lwe-HPLC yiloBheka i-valve. Yize kuvame ukunganakwa, i-valve yesheke idlala indima ebalulekile yokulawula ukuhamba kwesigaba seselula, ukugcina ubuqotho bohlelo, nokuvikela imishini ebucayi efana nephampu. Kulesi sihloko, sizohlola ukubaluleka kwama-valves okuhlola ezinhlelweni ze-HPLC, izinhlobo zabo, imisebenzi, kanye nokubaluleka kokulungiswa okufanele.
Indima ebalulekile yama-valves ahlola i-HPLC
I-valve yokuhlola ku-HPLC ivimbela ukubuyela emuva okungafuneki kwama-solves noma izigaba zeselula ohlelweni, kuqinisekisa ukuhamba okungaguquki nokuqondisa. Le nto elula nokho ebalulekile ibalulekile ekuqinisekiseni imiphumela enembile, yokuzalela ama-chromatographic. Nakhu ukubhekisisa okusondele emisebenzini ebalulekile ye-Check Valve:
1. Ukuvimbela ukubuyela emuva
Umsebenzi oyinhloko we-valve yesheke ukuvikela ubuyela emuva kwesigaba seselula noma solvent. Kuzinhlelo ze-HPLC, ukulondolozwa kwendlela yokugeleza njalo kubalulekile ukugwema ukungcola noma imiphumela engalungile. Ngaphandle kwe-valve yesheke, kungaba nengozi yokugeleza okuguqukayo, okungaphumela ekuxubeni kwezixazululo, ukungcoliswa kwamasampula, noma ukuhlukaniswa okungafanele kwamakhompiyutha.
2. Ukuvikela iphampu
Iphampu ye-HPLC iyingxenye ebalulekile yohlelo oluqinisekisa ukuthi isigaba seselula sihamba ngekholamu ngengcindezi edingekayo. Kodwa-ke, lapho iphampu limiswa, ingcindezi ingayehla, ibangele ukubuyela emuva. I-valve yokuhlola iqinisekisa ukuthi ingcindezi igcinwa noma ngabe iphampu ingasebenzi ngenkuthalo, ivimbela ukulimala kwephampu noma ukulahleka kwengcindezi.
3. Ukulondolozwa kobuqotho bohlelo
Izinhlelo ze-HPLC zincike ekulinganiseni okubucayi phakathi kwengcindezi, isilinganiso sokugeleza, kanye nokwakheka kwe-solvent. Uma indlela yokugeleza isengozini ngenxa yokubuyela emuva, ingahlehlisa lonke uhlelo. I-valve yesheke igcina ubuqotho bohlelo ngokuqinisekisa ukuthi isigaba seselula sigeleza kuphela endaweni oyifunayo kuphela, ngcono ukunemba nokuvumelana kokuhlaziywa.
Izinhlobo zama-valves ezihlolayo ezisetshenziswa ku-HPLC
Izinhlobo ezahlukene zama-valves ezihlolo zisetshenziswa ezinhlelweni ze-HPLC, ngamunye aklanyelwe ukuhambisana nezidingo ezithile zokusebenza. Nazi ezinye zezinhlobo ezivame kakhulu:
1. I-valve yokuhlola elayishwe entwasahlobo
I-valve yesheke ethwele entwasahlobo iyona esetshenziswa kakhulu ezinhlelweni ze-HPLC. Isebenzisa indlela yentwasahlobo yokuvala i-valve lapho kungekho ukugeleza noma lapho ukuqondiswa kokugeleza kubuyiselwa emuva. Lolu hlobo lwe-Check valve luthembekile futhi kulula ukulugcina.
2. I-valve ye-Ball Check
Kulomklamo, ibhola licindezelwa esihlalweni ukuvimbela ukubuyela emuva. Lapho ukugeleza kumisa, ibhola liveza i-valve, livimba noma yikuphi ukugeleza okubuyiselwe emuva. Ama-valves wokuhlola ibhola alula futhi asebenzayo, abenze isinqumo esithandwayo sezinhlelo ezincane ze-HPLC.
3. I-Diaphragm Check valve
I-valve yokuhlola i-diaphragm isebenzisa i-diaphragm eguquguqukayo ukubeka uphawu lwe-valve lapho kungazelelwe ukugeleza. Lolu hlobo lwe-valve lufanelekile ezinhlelweni ezidinga isesici esiphansi sengcindezi, njengoba i-diaphragm ingashintshanisa nezinguquko ezincane kwengcindezi.
Aphi ama-valves atholakala ezinhlelweni ze-HPLC?
Bheka ama-valves ngokuvamile abekwe ezindaweni ezinamasu ngaphakathi kohlelo lwe-HPLC ukuvimbela ukubuyela emuva ngamaphoyinti abalulekile. Lezi zindawo zingafaka:
•Ekhanda lempompo:Ama-valves ahlole avame ukutholakala emhlanganweni wamapompu ukuvikela ukugeleza okubuyela emuva kwe-solvent futhi ulondoloze ingcindezi engaguquki ngaphakathi kohlelo.
•Ku-Indertor:Kwezinye izinhlelo, ama-valves wokuhlola atholakala ku-injestor ukuvikela ukubuyela emuva ngesikhathi sokujova kwesampula, uqinisekise ukuthi isampula yethulwa kahle ohlelweni.
Ukubaluleka kokuhlola ukulungiswa kwe-valve
Njengazo zonke izingxenye ohlelweni lwe-HPLC, ama-valves wokuhlola adinga ukulungiswa njalo ukuze aqinisekise ukuthi asebenza kahle. Ngokuhamba kwesikhathi, ama-valves ahlole angavinjelwa ngama-concleates, acekelwa phansi ama-solvents, noma ahlangenwe nakho okugqokwa nokudabuka ngenxa yokusetshenziswa okuphindaphindiwe. Lokhu kungaholela ezindabeni ezinjengokuvuza, ukulahlekelwa kwengcindezi, noma ukugeleza okungahambelani. Ukuhlolwa njalo, ukuhlanza, kanye nokufakwa esikhundleni kwama-valves okuhlola kungavimbela lezi zinkinga, kuqinisekisa ubude be-HPLC yakho nokugcina ikhwalithi yemiphumela yakho.
Ngamafuphi, i-valve yokuhlola ohlelweni lwe-HPLC idlala indima ebalulekile ekulondolozeni ukugeleza okulungile kwesigaba seselula, ukuvimbela ukuvela emuva, nokuvikela izakhi ezibucayi njengepompo. Ngokuqonda umsebenzi wayo futhi ulondoloze le nto elula kodwa ebalulekile, ungathuthukisa ukunemba, ukusebenza kahle, nokuphila isikhathi eside kohlelo lwakho lwe-HPLC. Noma ngabe wenza okujwayelekile kuhlaziya noma usebenza emisebenzini eyinkimbinkimbi ye-Chromatographic, ungakunaki ukubaluleka kwe-valve esebenzayo esebenza kahle ekuqinisekiseni ukusebenza kahle kohlelo.
Ukulungiswa njalo kanye nokuqonda izinhlobo zama-valves ezihlolo ezitholakalayo kungasiza ukunciphisa izingqinamba futhi kuthuthukise ukuthembeka kohlelo lwakho lwe-HPLC.
Isikhathi sePosi: Nov-07-2024