Emhlabeni wokuhlaziywa kwamakhemikhali nokuhlolwa kwelebhu, ukunemba kubalulekile. Noma ngabe wenza i-chromatography noma okunye ukuhlaziya, ikhwalithi yemishini yakho inomthelela ngqo ekuthembekeni kwemiphumela yakho. Isakhi esisodwa esibucayi esivame ukunganakwa siyinto eyisampula ngaphakathiI-Agililent Autosampler Injerjectors. Le ngxenye encane kodwa ebalulekile iqinisekisa ukuthi amasampula afakwa ngokunembile ohlelweni, ethinta ukusebenza okuphelele kanye nokusebenza kahle kokuhlaziywa.
Kepha yini ngempela eyenza i-loop enhle yesampula, futhi kungani into yayo ebonakalayo inendaba kangaka? Kulesi sihloko, sizokwehla endimeni yama-loops wesampula, izinto ezisetshenzisiwe, nokuthi ungazikhetha kanjani izinketho ezinhle kakhulu zokusetha kwakho kwelebhu.
Yini i-loop yesampula futhi kungani ibalulekile?
I-loop yesampula iyingxenye encane, ye-tubulale ngaphakathi kohlelo lwe-Inductor oluphethe ivolumu eqondile yesampula ngaphambi kokuthi ifakwe ku-chromatograph noma ezinye izinsimbi zokuhlaziya. Inhloso yayo ukuqinisekisa ukuthi i-injeva yesampula yenzelwe ivolumu efanele, ethinta ngqo ukunemba kanye nokuvela kwemiphumela yokuhlolwa.
I-discurate isampula amavolumu angaholela kwimininingwane ebekiwe, eholela emaphutheni angaba khona ekuhlaziyweni futhi ekugcineni athinte imiphumela yocwaningo noma yokukhiqiza. Ngakho-ke, ukuqinisekisa ikhwalithi nokunemba kwe-loop yesampula kubalulekile ekutholeni imiphumela ethembekileko ezinqubweni zokuhlaziya.
Izinto zokwakha: insimbi engenasici vs Peek
Izinto ezisetshenziselwa ukwakha i-loop yesampula zingathinta kakhulu ukusebenza kwazo nokuphila isikhathi eside. Izinto ezimbili zezinto ezivame kakhulu ezisetshenziselwa ukwenza ama-loops wesampulainsimbi engagqwalina-I-Peek (Polytheketewoone). Ake sihlole ukuthi lezi zinto zihlukile kanjani nokuthi kungani ngamunye angafaneleka izidingo ezihlukile zelebhu.
I-Stinanless yensimbi yesampula
Insimbi engenasici kube yinto yokuya entweni yesampula yeminyaka eminingi. Yaziwa ngokuqina kwayo, ukumelana nokugqwala, kanye nekhono lokubekezelela ingcindezi ephezulu, insimbi engenasici inikeza ukusebenza okuhle kwezilungiselelo eziningi zelebhu. Isakhiwo saso esiqinile siqinisekisa ukuthi i-loop yesampula igcina ukwakheka nobuqotho bayo, ukunciphisa ubungozi bokuvuza nokulahleka kwesampula ngesikhathi sokujova.
Ngokwengeziwe, insimbi engagqwali iyamelana namakhemikhali ahlukahlukene, okwenza ilungele ukusetshenziswa ezinhlelweni ezahlukahlukene lapho ukuqina kwamakhemikhali kubalulekile. Kodwa-ke, ama-loops ensimbi engagqwali angeke alungele amasampula abucayi kakhulu noma izindawo ezidinga amazinga aphansi we-Ultra-aphansi, njengoba impahla kwesinye isikhathi ingadlulisela ama-metals wesampula.
I-Peek Sampula Loops
I-Peek yi-polymer ephezulu ukusebenza eyaziwa ngamandla wayo wokungena, amandla emishini, kanye nokumelana namazinga okushisa aphezulu. Ama-loops wesampuli enziwe kusuka ku-Peek azuzisa kakhulu ezinsizakalweni ezibucayi lapho kungcoliswa kwezinsimbi noma ezinye izinto ziyinkinga. Izakhiwo ze-peety i-ineert ziqinisekisa ukuthi ayisebenzisane nesampula, okwenza kube kuhle ngokuphatha amakhompiyutha aguqukayo noma asebenzayo.
Enye inzuzo ye-Peek ukuguquguquka kwayo kanye nesisindo esikhanyayo uma kuqhathaniswa nensimbi engenasici, okungenza kube lula ukuphatha ngesikhathi sokufakwa noma okunye. Kodwa-ke, i-Peek kungenzeka ingabekezeleli ingcindezi ephezulu kanye nensimbi engagqwali, ngakho-ke ukusetshenziswa kwayo kunconyelwe izinhlelo eziphansi zengcindezi.
Ungayikhetha kanjani i-loop yesampula efanelekile yesicelo sakho
Ukukhetha i-loop yesampula efanele kuncike ezicini eziningana, kufaka phakathi uhlobo lwesampula, uhlobo lokuhlaziywa, kanye nemvelo yokusebenza. Nazi izinto okufanele uzicabangele lapho ukhetha impahla ye-loop yakho yesampula:
1. Uhlobo lwesampula: Uma usebenza namasampula abucayi noma aguqukayo, i-Peek Sampula Loop kungenzeka ukuthi ukhetho olungcono ngenxa yemvelo yalo ye-inert. Kodwa-ke, ukuthola izicelo eziqinile noma zezimboni, ngensimbi engenacala kungaba inketho ehlala isikhathi eside.
2. Ukufaneleka kwamakhemikhali: Zombili lezi zinto zokwenziwa zinikeza ukumelana okuhle kumakhemikhali, kepha ngenxa yezimo zamakhemikhali ezedlulele, insimbi engenacala ingahle iphume. Njalo qiniseka ukuthi lokho okukhethayo kuyahambelana nama-solvents kanye nama-reagents asetshenziswe ekuhlaziyweni kwakho.
3. Izimo Zokucindezela: Uma uhlelo lwakho lusebenza ekucindezelweni okuphezulu, ngensimbi engenacala kungenzeka kube ukukhetha okungcono njengoba kungamelana nalezi zimo ngaphandle kokuyekethisa ubuqotho bayo.
4. Ukuqina: Insimbi engenasici iyinto ehlala isikhashana ngokwedlulele, ikakhulukazi izinhlelo ezidinga ukusetshenziswa kaningi. I-Peek, kuyilapho ihlala isikhathi eside, ingahle ingahlali isikhathi eside ngaphansi kokusetshenziswa kanzima noma izimo ezimbi kakhulu.
5. Usayizi nokuvumelana nezimo: Uma ukuguquguquka nokufaka kalula ukufakwa kubalulekile, ama-loops wesampula e-Peek anikeza inketho elula futhi eguquguqukayo. Ngakolunye uhlangothi, insimbi engagqwali, inikeza ngokuqinile, kwesinye isikhathi okungathenjwa ngokwengeziwe ezinhlelweni ezithile.
Ukugcina
Ama-loops wesampuli ayingxenye encane kodwa ebucayi emijondolo ye-agilent autosampler, kanye nokukhetha izinto ezifanele ze-loop yakho kubalulekile ekuqinisekiseni ukunemba, ukusebenza kahle, nokuphila isikhathi eside ezinhlelweni zakho zokuhlaziya. Noma ngabe ukhetha insimbi engagqwali noma ebheke phezulu, ukuqonda izinzuzo zento ngayinye kuzokusiza wenze isinqumo esinolwazi sezidingo zakho zelebhu.
Ngokutshala imali kuma-loops wesampula asezingeni eliphakeme futhi ugcina njalo imishini yakho, ungathuthukisa ukunemba kokuhlaziywa kwakho futhi uzuze imiphumela ethembekile ngaso sonke isikhathi. Uma usukulungele ukuhlola izihibe zesampula eziphezulu zelebhu yakho,ChromasirInikeza uhla lwezinketho zokusebenza okuphezulu ukuze uhlangabezane nezidingo zakho.
Isikhathi sePosi: Feb-20-2025