Izinhlobo Ze-Liquid Chromatography

Uma uhlela ukuthenga okokusebenza kwe-chromatography ewuketshezi yocwaningo lwaselabhorethri noma ukuhlolwa kwezimboni, ungase ukhathazwe uchungechunge lwemibuzo. Iluphi uhlobo lwe-chromatography ewuketshezi olufaneleka kakhulu kuzidingo zakho zokuhlaziya isampula, njengokuhlukanisa izinhlanganisela eziphilayo ezibilayo noma ukulandelela ama-molecule e-biological? Ungaqinisekisa kanjani ukuthi okokusebenza okukhethiwe kuhlangabezana nokunemba okuqinile kanye nezidingo ezizwelayo zemboni yakho? Futhi ingabe uwuqonda ngokucacile umehluko ezimeni zohlelo lokusebenza phakathi kwamamodeli ahlukene e-liquid chromatography, ukuze ugweme ukungafani phakathi kwemisebenzi yesisetshenziswa kanye nemisebenzi yokuhlola yangempela?

I-chromatography ewuketsheziubuchwepheshe bokuhlaziya obuyisisekelo obusetshenziswa kakhulu emikhakheni efana ne-biopharmaceuticals, ukuphepha kokudla, nokuqapha indawo ezungezile. Ukukwazi izinhlobo zayo ezithile nezici kubalulekile ekukhetheni imishini efanele. Okulandelayo kuzocacisa ngezinhlobo, izigaba zomkhiqizo womkhiqizo, izinzuzo, amamaki wezinto ezibonakalayo, nezinkambu zohlelo lokusebenza lwe-chromatography ewuketshezi ukukusiza wenze izinqumo ezinolwazi.

Izinhlobo Ezivamile Ze-Liquid Chromatography

Emakethe, i-chromatography eyi-liquid ihlukaniswa kakhulu ngezinhlobo ezimbalwa ngokususelwa kumigomo yokuhlukanisa kanye nezimo zohlelo lokusebenza. I-High-Performance Liquid Chromatography (HPLC) iwuhlobo olusetshenziswa kakhulu, olunokusebenza kahle kokuhlukana okuphezulu kanye nesivinini sokuhlaziya okusheshayo, esilungele ukutholwa okuningi kwenhlanganisela yezinto eziphilayo. I-Ultra-High Performance Liquid Chromatography (UHPLC) inokumelana nokucindezela okuphezulu nokuzwela okungcono, okungafinyeza isikhathi sokuhlaziya ngaphezu kuka-50% uma kuqhathaniswa ne-HPLC futhi ivamise ukusetshenziswa ezimweni zokuhlola komphumela. I-Two-Dimensional Liquid Chromatography (2D-LC) ihlanganisa amasistimu okuhlukanisa amabili ahlukene, enweba ububanzi bezinto ezibonakalayo futhi iyasebenza ekuhlolweni kukamatikuletsheni ayinkimbinkimbi njengokuchayeka kwe-serum exogenous. Ngaphezu kwalokho, kunezinhlobo ezikhethekile ezifana ne-Ion-Exchange Chromatography yokuhlukaniswa kwenhlanganisela ye-ionic kanye ne-Size-Exclusion Chromatography yokuhlaziywa kwe-macromolecular substance.

Izigaba ze-Maxi Scientific Liquid Chromatography

I-Maxi Scientific Instruments (Suzhou) Co., Ltd., egxile emkhakheni we-chromatography, inikeza uhla oluphelele lwemikhiqizo ehlobene ne-chromatography ewuketshezi. Imikhiqizo yayo eyinhloko ihlanganisa Amakholomu e-Ghost-Sniper asebenza ngokuqhathaniswa nemikhiqizo yasekuqaleni, izesekeli ze-PEEK HPLC, nama-capillary ensimbi engagqwali angu-316L. Le mikhiqizo inezinzuzo zamanani athengekayo, izikhathi ezimfushane zokulethwa, nokumelana nokugqwala okuqinile. Isibonelo, i-capillary yensimbi engagqwali engu-316L ithatha inqubo ekhethekile yokuvikela, engenakho kuphela ukusebenza okuhle kakhulu kokuvala kodwa futhi ingafakwa kalula ngesandla, efanelekile ukuhambisana nezinhlelo ezihlukahlukene ze-chromatography eziwuketshezi.

Ubuhle be-Liquid Chromatography

Mayelana nezinzuzo ezijwayelekile, i-chromatography ewuketshezi ingahlaziya u-80% wezinto eziphilayo, ikakhulukazi izinto ezibilayo kakhulu, ezingazinzi ngokokushisa, kanye nezinto ezisebenza ngokwebhayoloji okunzima ukuziphatha nge-gas chromatography. Ukuzwela kwayo kokutholwa kuphezulu, futhi umtshina we-ultraviolet ungafinyelela ku-0.01ng, ongahlangabezana nezidingo zokuhlaziya umkhondo.

Ezinhlotsheni ezivamile, i-HPLC inezinzuzo zamakholomu asebenziseka kabusha kanye nokusetshenziswa kwesampula encane; I-UHPLC inekhono elingcono lokuhlukanisa (okuphindwe kathathu kune-HPLC evamile) kanye nezinga eliphansi lokungcoliswa; I-2D-LC inganweba uhla lwe-coefficient yokuhlukanisa uwoyela namanzi ezinto ezibonakalayo ukuya ku-8 kuya ku-12, ibona ukubhekwa okuphezulu kokungcola okuningi.

Imikhiqizo ye-Maxi Scientific inezinzuzo ezihlukile. Ikholomu yayo ye-Ghost-Sniper yamukela inqubo yokukhiqiza eyingqayizivele, eqinisekisa ukusebenza okuzinzile kuyilapho kunciphisa izindleko. Imikhiqizo ye-capillary inempilo ende yesevisi, enganciphisa imvamisa yokushintshwa kwemishini kubasebenzisi futhi yonge izindleko zokusebenza.

Amabanga we-Liquid Chromatography Material

Izingxenye eziyinhloko ze-chromatography ye-Liquid zinezindinganiso zezinto ezibonakalayo eziqinile. Uma sithatha ikholomu njengesibonelo, isigaba esimile sisebenzisa izinhlayiya ezinezimbotshana ezinosayizi wezinhlayiyana ezingu-5-10μm ze-HPLC kanye nezinhlayiya ezincane ze-UHPLC ukuze kuthuthukiswe ukusebenza kahle kokuhlukana. Ipayipi ngokuvamile lenziwe ngensimbi engagqwali engu-316L (imelana nokugqwala) noma impahla ye-PEEK (efanele amasampula e-asidi eqinile ne-alkali).

Ngokwamazinga ebanga lemboni, okokusebenza kudinga ukuhlangabezana nezinkomba zokusebenza ezifana nokunemba kwezinga lokugeleza (±1% noma ±2μL/min) nokuzinza kokulawula izinga lokushisa (±0.1℃). Isibonelo, imikhiqizo ye-Maxi Scientific ithobelana nezitifiketi ze-ISO9001:2015 zokuphathwa kwekhwalithi, iqinisekisa ukuthi ikhwalithi yomkhiqizo ihlangabezana nezindinganiso zamazwe ngamazwe.

Izicelo ze-Liquid Chromatography

Emkhakheni we-biopharmaceuticals, i-chromatography ewuketshezi isetshenziselwa ukuhlanzwa kwamaprotheni nokulawula ikhwalithi yezidakamizwa. Isibonelo, ingahlukanisa futhi ibone ama-amino acid nama-peptide kumasampula ebhayoloji. Ekuhloleni ukuphepha kokudla, ingahlaziya izithasiselo zokudla njengezilondolozi kanye nokungcola okufana nezinsalela zezibulala-zinambuzane, ezinomkhawulo wokutholwa ophansi njengamaleveli okulandelela. Ekuqaphelweni kwemvelo, isetshenziselwa ukuthola ukungcola kwemvelo njengama-polycyclic aromatics hydrocarbons nama-phenol emanzini nasenhlabathini.

Imikhiqizo ye-Maxi Scientific isetshenziswe emikhakheni eminingi. Kuphrojekthi yokuhlaziya ukudla, Ikholomu yayo ye-Ghost-Sniper iqedele ngempumelelo ukuhlukaniswa nokutholwa kwezithasiselo zokudla eziningi, ngezinga lokuthola kabusha elingaphezu kuka-95% kanye nedatha ezinzile. Kuphrojekthi yokuhlola imvelo, i-capillary yensimbi engagqwali engu-316L ehambisana nohlelo lwe-chromatography ewuketshezi yabona ukuqapha okuqhubekayo kwamasampula amanzi amahora angama-240, iqinisekisa ukunemba kwemiphumela yokuhlolwa.

Isiphetho

I-Liquid chromatography inezinhlobo eziningi ezifana ne-HPLC, i-UHPLC, ne-2D-LC, ngayinye enezinzuzo eziyingqayizivele nezimo ezisebenzayo. Imikhiqizo ehlobene ne-chromatography ye-liquid ye-Maxi Scientific, esebenza kahle kakhulu, ifinyeleleka kalula, kanye nekhwalithi ethembekile, ingahlangabezana nezidingo zezimboni ezahlukene. Noma ngabe wenza ucwaningo lwe-biopharmaceutical, ukuhlola ukuphepha kokudla, noma ukuqapha indawo ezungezile, ukukhetha imikhiqizo ye-Maxi Scientific kungakusiza uthuthukise ukusebenza kahle kokuhlaziya futhi wehlise izindleko. Manje, sicela uthinte i-Maxi Scientific ngokushesha (shayela ku-+86 400-6767580) ukuze uthole izingcaphuno zomkhiqizo nezinsizakalo zokubonisana zangaphambi kokuthengisa!