Ungawengeza kanjani ukuhlaziywa kwe-HPLC futhi uthuthukise ukusebenza kwelabhothi

Ema-laboratorics wokuhlaziya,I-Chromatography ephezulu ye-chromatography (HPLC)kuyindlela ebalulekile yokuhlukanisa, ukukhomba, kanye namakhompiyutha ancomekayo. Kodwa-ke, ukufeza imiphumela engaguquki futhi ethembekile kudinga ngaphezu kwemishini efanelekile kuphelaukubimba. Lo mbhalo uhlola ukuthi ungakuthuthukisa kanjani okwakhoUkuhlaziywa kwe-HPLCUkuze wandise ukusebenza kahle, ukunciphisa isikhathi sokuphumula, futhi uthuthukise ukunemba.

Izinselelo ezijwayelekile ekuhlaziyweni kwe-HPLC nokuthi ungazixazulula kanjani

Ngenkathi i-HPLC iyithuluzi elinamandla lokuhlaziya, alikho ngaphandle kwezinselelo. Izingqinamba ezinjengokuthiIsinqumo esingesihle, umsindo wesisekelo, nemiphumela engahambelanikungaphazamisa ukusebenza kwelabhothi. Nakhu ukuthi ungabhekana kanjani nalezi zinkinga ezivamile:

1. Ukulungiswa kabi

Enye yezinkinga ezivame kakhulu ku-HPLC ukuhlukaniswa okumpofu phakathi kweziqongo, kaningi ngenxaUkukhethwa okungalungile ikholomu noma amanani okugeleza okungaphansi kwe-suffoptimal. Ukwenza ngcono ukulungiswa:

• Khetha aikholomu ye-chromatographicNgokufanelekileIsigaba se-Static Station kanye nosayizi wezinhlayiyanakokuhlaziywa kwakho.

• lungisaIzinga lokugeleza nezimo ze-gradientukuthuthukisa ubukhali obukhulu kanye nokwahlukana.

• sebenzisaUkulawulwa kokushisaukuqinisa izikhathi zokugcina futhi uthuthukise ukuvela kabusha.

2. I-Baseline Drift noma umsindo

Umsindo wesisekelo ungaphazamisa ukutholwa kwe-Peak futhi uyeke ukunemba kwedatha. Le ndaba ivame ukubangelwa yi:

•Ukushisa Ukushintshashintsha- Gcina indawo ezinzile elebhu bese usebenzisa i-oveni yekholomu uma kunesidingo.

•Isigaba seselula esingcolisiwe- Sebenzisa izixazululo eziphezulu zokuhlanzeka bese uhlunga isigaba sakho seselula ngaphambi kokusetshenziswa.

•Ukungcoliswa Kwensimbi- Hlanza njalo futhi ulondoloze umtshina, ipompo, neshubhu ukunciphisa umsindo wasemuva.

3. Ukuhlanganiswa Okungahambelani Okuphezulu

Ukuhlanganiswa okungahambisani kuthinta ukwethenjwa kokuncishiswa. Ukuxazulula lokhu:

• qinisekisaIkholomu le-HPLC linesimo esifanelengaphambi kokusebenzisa.

• Gcina aIzinga eliqinile lokugelezafuthi uvikele ukuguquguquka kwengcindezi.

• lungisaIzilungiselelo zesoftware zokuhlanganiswa okuphezulu, eqinisekisa imiphumela engaguquguquki futhi engaguquki.

Ukukhetha ikholomu ye-HPLC efanele

Ukukhetha ikholomu efanele ye-HPLCkubalulekile ukufeza ukwahlukana okuhle. Cabanga ngalezi zinto lapho ukhetha ikholamu:

•Ubude bekholomu: Amakholomu amade ahlinzeka ngokwahlukana okungcono kepha akhuphule isikhathi sokuhlaziywa. Khetha ubude bokuxazululwa kwezilinganiso kanye nesivinini.

•Ububanzi bekholomu: Amakholamu ama-nlamthamo anikela ngokulungiswa okuphezulu kepha adinga ingcindezi engaphezulu. Qinisekisa ukuhambisana nohlelo lwakho lwe-HPLC.

•Isigaba sokumaphakathi: Khetha isigaba nge-chemistry efanelekile yokuhlaziywa kwakho (isib., C18 nge-Compounds Engelora, Phenyl ye-Aromatic Compounds).

Ukunxusa izigaba zeselula namanani wokugeleza

Isigaba seselula siyisihluthulelo sokuhlaziywa okuphumelelayo kwe-HPLC. Nakhu ukuthi ungakukhulisa kanjani:

•Lungisa ukwakheka kwe-solvent: Fine-Tune TheIsilinganiso solventukwenza ngcono ukuhlukaniswa. Umsebenziukuxwaya kweziqukumasampula ayinkimbinkimbi.

•Lawula amazinga we-PH: QinisekisaIsigaba seselula PHiyahambisana nesampula nekholamu.

•Lungiselela izinga lokugeleza: Amanani okugeleza aphezulu anciphisa isikhathi sokuhlaziywa kepha angahlehlisa isisombululo. Ijubane elilinganiselayo kanye nokusebenza kahle kususelwa kwindlela yakho.

Ukulungiswa nokunakekelwa kokuvimbela

Isondlo esifaneleUkusebenza okungaguquki futhi kunwebeka isikhathi sokuphila kwensimbi. Landela lezi zindlela ezinhle kakhulu:

•Ukuhlanza okujwayelekile: Hlanza njaloI-Inder, ikholamu, kanye nomhloliukuvikela ukungcoliswa.

•Faka okunye okudliwayo: Shintshaizimbotshana, izihlungi, kanye tubingnjengoba kudingeka ukuvikela ukushintshashintsha kwengcindezi.

•Alinganise uhlelo: Ukulinganisa njalo ukudonsa kwangaphansi nezinye izinto ezibucayi ukuqinisekisa imiphumela efanele.

Ukugcina

Ukulungiswa kokuhlaziywa kwe-HPLC kubalulekile ekwenzeni ngcono ukusebenza kwelabhothi kanye nokuqinisekisa imiphumela ephezulu. Ngokubhekana nezinkinga ezijwayelekile ezifanaIsinqumo esingezinhle, umsindo wesisekelo, kanye nokuhlanganiswa okuhlanganisiwe, nangokukhetha ilungeloAmakholomu nezigaba zeselula, ungathuthukisa kakhulu ukusebenza kwakho kokuhlaziya. -Njaloukulungiswa nokusebenzisa kahle kwendlelaIzogcina uhlelo lwakho lwe-HPLC lusebenza ngokusebenza kahle, ukunciphisa isikhathi sokuphumula kanye nokuqinisekisa imiphumela enembile, ephindeka.

Ngokuholwa uchwephesheUkusebenza kwe-HPLC, thintanaChromasir-Sikhethekile ekuhlinzekeniIzixazululo ezenziwe ngokwezifiso ze-ChromatographyUkusiza elabhoratri yakho ukufeza amazinga aphezulu okusebenza.