I-Chromatography iyindlela esemqoka ocwaningweni lwesayensi yesimanje, kodwa ukuvela kweiziqongo zezipokikuma-chromatogram kungadala izinselele ezibalulekile kubahlaziyi. Lezi ziqongo ezingalindelekile, ezivame ukuvela ngesikhathi sokuhlukaniswa kwe-chromatographic, ikakhulukazi kumodi ye-gradient, ziphazamisa ukuhlaziya ubuningi futhi zimoshe isikhathi esibalulekile. Ukuqonda izimbangela zokukhuphuka kwezipoki kanye nokusebenzisa izixazululo ezintsha njenge-Chromasir'sIkholomu ye-Ghost-Sniperingaguqula izinqubo zakho zokuhlaziya.
Ziyini Iziqongo Zomoya futhi Kungani Zibalulekile?
Ukuphakama kwesipoki amasignali angaziwa kuchromatogram aphazamisa ukucaca kwemiphumela yokuhlaziya. Nakuba zingase zibonakale zincane, umthelela wazo ungaba mkhulu:
1. Izinselele zobuningi
Uma izipoki eziphakeme zidlulela neziqongo zentshisekelo, zenza kube nzima ukulinganisa okunembile kwabahlaziyi. Lokhu kungaholela ekuchazeni idatha okunephutha kanye nemiphumela engathembekile.
2. Ukuxazulula Inkinga Okudla Isikhathi
Ukuhlonza umthombo wezipoki ngokuvamile kudinga uphenyo olude, kuphambukise ukugxila kwabahlaziyi emisebenzini ebalulekile. Isikhathi esichithwa ekuxazululeni lezi zinkinga singathuthukisa umkhiqizo kanye nemiphumela yocwaningo.
Ingabe I-Ghost Peaks ivelaphi?
Ukuze kuqedwe ngempumelelo iziqongo zezipoki, kubalulekile ukuqonda imvelaphi yazo. Lezi ziqongo ezingalindelekile ngokuvamile zivela kukungcola oku:
1.Izingxenye Zesistimu:Izinsalela ohlelweni lwe-chromatographic zinganikela ekuphakameni kwezipoki.
2.Amakholomu:Ukungcola kwempahla yokupakisha noma ukuguga kokusetshenziswa kungase kubangele ukungcola.
3.Amasampuli:Amasampuli angcolile ethula izinhlanganisela ezingalindelekile kuchromatogram.
4.Isigaba samaselula:Ukungcola okuvela ezincibilikilweni, usawoti oyisivikelo, noma izigaba ezinamanzi/eziphilayo ngokuvamile zinomthelela ekuphakameni kwezipoki.
5.Iziqukathi:Amabhodlela okulungiselela amasampula nezinye iziqukathi zingaletha ukungcola okusele.
Isixazululo Senguquko: Ikholomu Ye-Ghost-Sniper
I-Chromasir'sIkholomu ye-Ghost-Sniperiyisixazululo esishintsha umdlalo esiklanyelwe ukulwa neziqongo zezipoki. Le kholomu yesizukulwane sesibili inesakhiwo esithuthukisiwe nezinto zokupakisha ezithuthukisiwe, eziyenza ikwazi ukuthwebula kahle iziqongo eziyizipoki ngisho nangaphansi kwezimo ezimbi kakhulu. Ukusebenza kwayo kuyenza ibe yithuluzi elibalulekile lokuqinisekisa indlela nokuhlaziywa komkhondo.
Amalabhorethri asebenzisa Ikholomu Ye-Ghost-Sniper abike ukuthuthuka okuphawulekayo kukhwalithi ye-chromatogram, izikhathi ezincishisiwe zokuxazulula izinkinga, kanye nokukhiqiza okuthuthukisiwe kukonke.
Uzikhulisa Kanjani Izinzuzo Zekholomu Ye-Ghost-Sniper
Ukuze usebenze kahle, landela lezi zinyathelo zokuphepha nezindlela ezihamba phambili:
1.Ukulungiswa Kwesikhathi Sebhalansi:
Engeza imizuzu engu-5–10 yesikhathi sebhalansi ezinhlelweni ze-HPLC ukuze uhambisane nevolumu yekholomu.
2.Ukusethwa Kwasekuqaleni:
Hlanza amakholomu amasha nge-acetonitrile engu-100% ngesilinganiso sokugeleza esingu-0.5 mL/min amahora angu-4 ngaphambi kokusetshenziswa ukuze uqinisekise ukusebenza okuhlanzekile nokusebenza kahle.
3.Phatha ama-Ion-Pair Reagents ngokuqapha:
Ama-reagents e-ion-pair esigabeni seselula angashintsha izikhathi zokugcinwa kanye nokuma okuphezulu. Sebenzisa ngokucophelela lapho ama-reagents anjalo ekhona.
4.Shintsha Amakholomu Njalo:
Ukuphila kwekholomu kuncike ezintweni ezifana nokuhlanzeka kwesigaba seselula, izimo zencibiliki, nokuhlanzeka kwemishini. Ukushintshwa okuvamile kuqinisekisa imiphumela engaguquki.
5.Izigaba zeselula eziqukethe usawoti we-Flush:
Sebenzisa isixazululo sesigaba se-organic esingu-10% (isb, i-methanol noma i-acetonitrile) ngaphambi nangemva kokuqalisa izigaba zamaselula ezinosawoti ukuze uvimbele ukuvinjwa.
6.Gcina kahle ngesikhathi sokuphumula:
Ukuze uthole isitoreji sesikhathi eside, gcina ikholomu ku-70% ye-organic aqueous solution (i-methanol noma i-acetonitrile). Hlanza nge-acetonitrile engu-100% ngaphambi kokuphinda uyisebenzise ukuze ubuyisele ukusebenza.
7.Gada ukusebenza:
Shintshanisa ikholomu uma umphumela wayo wokuthwebula uncipha noma uma izimfuno zokuhlaziya zidlula amandla ayo.
Kungani Amakholomu E-Ghost-Sniper Abalulekile Elebhu Yakho
Ikholomu ye-Ghost-Sniper ingaphezu kwethuluzi lokuxazulula izinkinga; ithuthukisa ukusebenza okuphelele kwesistimu yakho ye-chromatographic.
•Iqeda I-Ghost Peaks:Ngisho nasezimweni eziyinselele kakhulu, le kholomu ibamba iziqongo zezipoki ngempumelelo.
•Ivikela Izisetshenziswa:Ihlunga izinhlayiya eziqinile nezinto ezingcolisayo eziphilayo, ivikela amathuluzi namakholomu e-chromatographic.
•Ithuthukisa Ikhwalithi Yedatha:Ngokususa iziphazamiso, ikholomu ikhiqiza ama-chromatogram ahlanzekile, anokwethenjelwa.
Ama-Maxi Scientific Anstruments: Uzakwethu Ekuhlaziyeni Okuhle kakhulu
At I-Maxi Scientific Instruments (Suzhou) Co., Ltd., sihlinzeka ngezixazululo ezintsha zokunika amandla amalabhorethri emhlabeni wonke. Amakholomu ethu e-Ghost-Sniper aklanywe ngokucophelela ukubhekana nezinselele zezipoki eziphakeme, ukuqinisekisa imiphumela enembile kanye nokugeleza komsebenzi okushelelayo.
Thuthukisa i-Chromatography Yakho Namuhla
Ungavumeli ukuphakama kwezipoki kuphazamise ucwaningo lwakho. Faka imali kumakholomu e-Chromasir's Ghost-Sniper futhi uzwe umehluko wokunemba, ukusebenza kahle, nokuthula kwengqondo. Oxhumana nayeI-Maxi Scientific Instruments (Suzhou) Co., Ltd.namuhla ukuze ufunde kabanzi mayelana nokuthi izixazululo zethu zingakhuphula kanjani ukusebenza kwelabhorethri yakho. Ndawonye, masifinyelele ubuhle kuyo yonke ichromatogram.
Isikhathi sokuthumela: Dec-13-2024