I-Liquid chromatography hlola i-valve cartridge ruby ​​ceramic replacement Waters

Nini ukufaka i-valve yesheke?
① "I-Lost Prime" evela lapho isistimu isebenza ibonisa ukuthi ingcindezi yesistimu iphansi kakhulu, iphansi kakhulu kunokucindezela kwasemuva okudingekayo ekusebenzeni okujwayelekile kwe-chromatography yoketshezi. Ikakhulukazi ibangelwa ukungcoliswa kwe-valve yokuhlola ekhanda lepompo, noma amabhamuza amancane asala ku-valve yokuhlola okuholela ekufakweni okungabusheleli. Ngalesi sikhathi, okudingeka sikwenze ukwenza umzamo wokususa amabhamuza amancane ngokusebenzisa imizuzu emihlanu yokusebenza kwe-"Wet Prime". Uma lesi sixazululo sehluleka, kufanele sikhiphe i-valve yokuhlola, futhi siyihlanze nge-ultrasonically ngamanzi angaphezu kuka-80℃. Kunconywa ukufaka esikhundleni se-valve cartridge uma ukuhlanza okuphindaphindiwe kungasebenzi.

② Kuvele ukuthi kukhona amabhamuza ekhanda lepompo noma i-valve yokuhlola lapho ingcindezi yesistimu ishintshashintsha kakhulu. Singasebenzisa "I-Wet Prime" imizuzu engu-5-10, ukuze sikhiphe ama-bubbles ngesilinganiso sokugeleza okuphezulu. Uma indlela engenhla ingasebenzi, kufanele sikhiphe i-valve yokuhlola, futhi siyihlanze nge-ultrasonically ngamanzi angaphezu kuka-80℃. Kunconywa ukufaka esikhundleni se-valve cartridge uma ukuhlanza okuphindaphindiwe kungasebenzi.

③ Uma kunenkinga ngokukhiqizwa kabusha komjovo wesistimu, qala ngokubheka isikhathi sokugcina. Uma kunenkinga ngesikhathi sokugcina, hlola ukushintshashintsha kwengcindezi yesistimu kujwayelekile noma cha. Ngokuvamile, ngesilinganiso sokugeleza esingu-1ml/min, ukucindezela kwesistimu yensimbi kufanele kube ngu-2000~3000psi. (Kunomehluko wesilinganiso kuye ngezinhlobo zamakholomu echromatographic nezigaba zamaselula.) Kuvamile ukuthi ukushintshashintsha kwengcindezi kungaphakathi kuka-50psi. Ukushintshashintsha kokucindezelwa kwesistimu okulinganiselwe nokuhle kungaphakathi kuka-10psi. Ngaphansi kwesimo sokuthi ukushintshashintsha kwengcindezi kukhulu kakhulu, sidinga ukucabangela ukuthi kungenzeka yini ukuthi i-valve yokuhlola ingcolile noma inamabhamuza, bese sibhekana nayo.

Ungayisebenzisa nini i-valve yokuhlola i-ceramic?
Kunenkinga yokuhambisana phakathi kwe-ruby check valve ye-2690/2695 kanye nemikhiqizo ethile ye-acetonitrile. Isimo esiqondile siwukuthi: uma usebenzisa i-acetonitrile engu-100%, uyishiya ubusuku bonke, futhi uqhubeka nokuqala ukuhlolwa ngakusasa, akukho uketshezi oluphuma epompo. Lokhu kungenxa yokuthi umzimba wevalvu yokuhlola i-ruby kanye nebhola le-ruby kuhlanganiswe ndawonye ngemva kokucwiliswa ku-acetonitrile ehlanzekile. Kufanele sisuse i-valve yokuhlola futhi siyishaye kancane noma siphathe nge-ultrasonically. Lapho unyakazisa i-valve yokuhlola futhi uzwa umsindo omncane, lokhu kusho ukuthi i-valve yokuhlola ibuyela kokujwayelekile. Manje buyisela i-valve yokuhlola. Ukuhlolwa kungenziwa ngokujwayelekile ngemuva kwemizuzu emi-5 "ye-Wet Prime".

Ukuze ugweme le nkinga ekuhloleni okulandelayo, kunconywa ukusebenzisa i-valve yokuhlola i-ceramic.