Ikholomu ye-Ghost-Sniper Chromasir HPLC UPLC isusa iziqongo zezipoki

Ikholomu ye-Ghost-Sniper Chromasir HPLC UPLC ikholomu susa iziqongo zezipoki Isithombe Esifakiwe

incazelo emfushane:

Ikholomu ye-Ghost-Sniper iyithuluzi elinamandla lokuqeda ukuphakama kwezipoki ezikhiqizwa ngesikhathi senqubo yokuhlukaniswa kwe-chromatographic, ikakhulukazi kumodi ye-gradient. Ukuphakama kwezipoki kuzodala izinkinga zobuningi uma ukuphakama kwesipoki kudlula iziqongo zentshisekelo. Ngekholomu ye-Chromasir ghost-sniper, zonke izinselele zokuphakama kwezipoki zingaxazululwa futhi izindleko zokusetshenziswa kokuhlolwa zingancipha kakhulu.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Ikholomu ye-Ghost-sniper yenzelwe ukuqeda ukuphakama kwezipoki. Iziqongo zesipoki ziyimvelaphi engaziwa ku-chromatogram, ngokuvamile ebonwa phakathi nenqubo yokuhlukaniswa kwe-chromatographic ekukhanyeni kwe-gradient noma ukusebenza kwesikhathi eside. Ukuvela kweziqongo zezipoki kunethonya elijulile ekuhloleni kwabahlaziyi. Ikholomu ye-Ghost-sniper yenzeka ukuze ixazulule lezo zinkinga futhi yenze inqubo yokuhlola ibe lula futhi isebenze kahle. Le kholomu ingasetshenziswa esimweni esibucayi futhi ibonisa umphumela omuhle wokuthwebula. Impela kuyindlela enhle yokuqeda ukuphazamiseka okuvela eziqongweni zezipoki ekuqinisekiseni indlela kanye nokulandelela ukuhlaziywa kwezidakamizwa.

Amapharamitha

Ingxenye No.	Ubukhulu	Ivolumu	Isicelo
I-MC5046091P	50×4.6mm	cishe 800ul	I-HPLC
I-MC3546092P	35 × 4.6mm	cishe 580ul	I-HPLC
I-MC5021093P	50×2.1mm	cishe 170ul	I-UPLC
I-MC3040096P	30×4.0mm	cishe 380ul	Ivolumu yekholomu ephansi ye-HPLC

Ukufakwa

Isicelo Nemiphumela

Izinyathelo zokuzivikela

1. Uma ukuhlaziywa kwenqwaba kuhlelwa ohlelweni lwe-HPLC, kunconywa ukuthi ukhuphule isikhathi sebhalansi esengeziwe cishe amaminithi angu-5 - amaminithi angu-10 ezimeni zakho ze-chromatographic, ngenxa yethonya levolumu yekholomu ye-ghost-sniper.
2. Ukuze uthole amakholomu amasha, wageze nge-acetonitrile engu-100% ngenani lokugeleza elingu-0.5ml/min amahora angu-4 ngaphambi kokusetshenziswa.
3. Ama-reagents e-ion-pair esigabeni seselula, angase amuncwe ikholomu ye-ghost-sniper, engase ibe nomthelela esikhathini sokugcina kanye nokuma okuphezulu kwethagethi yakho. Sicela usebenzise ngokuqapha ngaphansi kwesigaba esinjalo seselula.
4. Ukuphila kwekholomu kuncike ezimweni zokuhlaziya, njengesigaba seselula. ukuhlanzeka kwe-solvent, kanye nokungcoliswa kwezinto zokusebenza. Sicela ushintshe ikholamu yesipoki-sniper njalo ukuze uqinisekise ukusebenza.
5. Ukushintsha ikholomu ye-ghost-sniper kuyaphakanyiswa uma umphumela wokuthwebula uba mubi kakhulu noma izimfuno zingenakwaneliswa.
6. Njengengxenye yokuhlanza ku-chromatograph ewuketshezi, ikholomu yesipoki-sniper ingahlunga izinhlayiya eziqinile futhi isuse ukungcola kwezinto eziphilayo ngaphambi komjovo. Ikholomu ye-ghost-sniper iphinde inikeze amathuluzi nekholomu ye-chromatographic ngokuvikela okungcono, futhi yenza kahle i-chromatogram.
7. Uma isigaba esihambayo siqukethe isinqamuli sikasawoti, ngaphambi nangemva kokuwusebenzisa, sidlulisele ku-10% isisombululo sesigaba se-organic (10% se-methanol noma i-acetonitrile), ukuze ugweme ukuphuma kukasawoti esibhafa futhi uvimbe ikholomu.
8. Sicela uqaphele ukuthi akuzona zonke iziqongo zezipoki ezingathwetshulwa ikholamu ye-Ghost-Sniper.
9. Uma ikholomu ishiya ingasetshenziswa isikhathi eside, kunconywa ukuyigcina kusixazululo se-organic aqueous (70% methanol noma i-acetonitrile). Futhi sicela uyihlanze nge-acetonitrile engu-100% ngesilinganiso sokugeleza esingu-0.5 ml/min ihora elingu-1 ngaphambi kokuyisebenzisa.